Journal: Molecular cell

Article Title: Phosphorylated RB Promotes Cancer Immunity by Inhibiting NF-κB Activation and PD-L1 Expression

doi: 10.1016/j.molcel.2018.10.034

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit polyclonal anti-Phospho-Rb (Ser795) , Cell Signaling Technology , Cat# 9301S; RRID: AB_330013.

Techniques: Virus, Recombinant, Control, Mutagenesis, Kinase Assay, Sequencing, CRAfT Assay, Software

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Effects of Annurca Flesh Apple Polyphenols in Human Thyroid Cancer Cell Lines

doi: 10.1155/2022/6268755

Figure Lengend Snippet: Effects of A FPE treatment on proliferation and viability of anaplastic thyroid cancer cell line. (a) Cell counting was performed by trypan blue reagent after treatment with increasing doses of A FPE for 24 hours. Black bars represent the viable cells (assessed by trypan blue exclusion), and dashed bars represent the dead cells. (b) The percentage of cell viability (viable cells/total cells) was calculated in CAL62 cell line treated with A FPE and counted in trypan blue dye. (c) Cell cycle analysis after propidium iodide (PI) staining of CAL62 untreated (NT) or treated with 250 or 500 μ M of A FPE EqC for 24 hours. Representative fluorescence-activated cell sorting (FACS) plots are shown applying the Michael Fox algorithm. (d) Protein extracts from CAL62 cells treated with 500 μ M of A FPE EqC for 24 hours and untreated were analyzed by Western blot using antibodies for phospho-Rb (Ser795) and for α -tubulin. Representative figures from at least four independent experiments are shown (left panel). Relative expression level of phospho-protein was calculated using α -tubulin as an endogenous control (right panel). Values are expressed as mean of at least three different experiments ± standard error of the mean (SEM). ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001 versus control (NT).

Article Snippet: Protein lysates were separated in a sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS-PAGE); then, the nitrocellulose membranes were hybridized with the following antibodies: rabbit polyclonal phospho-Rb (Ser795) diluted 1 : 1000 (#9301, Cell Signaling, Danvers, USA) and mouse monoclonal anti- α -Tubulin diluted 1 : 10000 (T-9026, Sigma-Aldrich, St. Louis, USA).

Techniques: Cell Counting, Cell Cycle Assay, Staining, Fluorescence, FACS, Western Blot, Expressing, Control

Journal: Cell chemical biology

Article Title: Discovery of covalent CDK14 inhibitors with pan-TAIRE family specificity

doi: 10.1016/j.chembiol.2019.02.015

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit polyclonal anti Phospho-RB S795 , Cell Signaling Technologies , Cat # 9301.

Techniques: Virus, Recombinant, Kinase Assay, Sequencing, Software